Journal: The EMBO Journal
Article Title: GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1 + tendon sheath progenitors
doi: 10.1038/s44318-025-00553-7
Figure Lengend Snippet: ( A , B ) Immunofluorescence staining and statistical analysis of the GLI1 + cells in injured tendons of Gli1-CreER T2 ; Ai9 and Gli1-CreER T2 ; Ai9; DTA mice at 21 dpi ( n = 5 per group). **** P = 1.74e-7. Scale bar, 200 μm. ( C , D ) Immunofluorescence staining and statistical analysis of the TPPP3 + cells in injured tendons of Gli1-CreER T2 and Gli1-CreER T2 ; DTA mice at 7 dpi ( n = 5 per group). **** P = 2.52e-5. Scale bar, 200 μm. ( E , F ) Immunofluorescence staining and statistical analysis of the SOX9 + cells in injured tendons of Gli1-CreER T2 and Gli1-CreER T2 ; DTA mice at 7 dpi ( n = 5 per group). *** P = 1.70e-4. Scale bar, 200 μm. ( G – I ) Statistical analysis of the RUNX2 + ( G ), COL2 + ( H ) and OPN + ( I ) cells in injured tendons of Gli1-CreER T2 and Gli1-CreER T2 ; DTA mice at 7 dpi, 21 dpi and 63 dpi, respectively. ( n = 5 per group). P values from left to right: *** P = 7.22e-4, **** P = 3.24e-6, **** P = 3.54e-5. ( J – L ) Immunofluorescence staining of the RUNX2 + ( J ), COL2 + ( K ) and OPN + ( L ) cells in injured tendons of Gli1-CreER T2 and Gli1-CreER T2 ; DTA mice at 7 dpi, 21 dpi and 63 dpi, respectively. Scale bar, 200 μm. Data is presented as mean ± SD. All P values were determined by unpaired Student’s t test.
Article Snippet: Anti-mouse TPPP3 , Proteintech , Cat#15057-1-AP.
Techniques: Immunofluorescence, Staining